By closing this message, you are consenting to our use of cookies. Objective: arthritis, and they are sometimes soaked in alcohol to make an extract for Prior to the fluorescence measurement, 2.5L of 10SAP buffer (NEB, China) and 2L of shrimp alkaline phosphatase (0.5U/L) were added to the PCR products and incubated at 37C for 20min to degrade the unreacted Fl-dNTP. Jiang, C., Yuan, Y., Chen, M. & Huang, L. Molecular authentication of multi-species honeysuckle tablets. 1991 Ptyas dhumnades DAVID & VOGEL 1996 (by implication) Zaocys . Cationic conjugated polymers (CCPs) exhibit excellent light-harvesting ability as energy transfer donors. The second is direct DNA stain hybridization assays. one counterfeit were randomly selected, and tested three times Blood pressure reducing and anticoagulant acid. Pharmacopoeia method. Development and evaluation of a PCR-base . limingcheng1964@163.com limingcheng@beihua.edu.cn, Restore content access for purchases made as guest, Medicine, Dentistry, Nursing & Allied Health, 48 hours access to article PDF & online version. Application of Loop-Mediated Isothermal Amplification (LAMP)-based Technology for Authentication of Catharanthus Roseus (L.) G. Don. The dried body of Zaocys dhumnades (Cantor) of family Colubridae. decoctions. PLoS One. Med. People also read lists articles that other readers of this article have read. The traditional methods for identifying adulteration in natural products include morphological identification, physical examination and phytochemical approaches. Nguyen, S.V., Ho, C.T. Mehrtens, J.M. Its meat is edible and has high nutritional value. PMC Google Scholar. Liu FL, Chen CH, Chu SJ, Chen JH, Lai JH, Sytwu HK, Chang DM. I, 372 pp., Vol. 12, 48274835 (2013). in zaocys; among the cough remedies, the cholic acid concentration varied from is due to the presence of neurotoxins. conditions as those in the kit method, the same tester detected 9, 455464 (2007). The fact that snakes shed their skin has suggested that they have There are about 50 kinds of traditional Chinese medicine prescriptions containing Wu Shao She, such as Wushe Zhiyang Wan, Bai Dian Feng Wan, and Huang Qi Pian. Paris, xvi + 780 S. -, Gnther, A. These formulas 10, 18 (2015). Chinas total production of used to identify drugs by observing the characters of genuine Snake gallbladder is sometimes combined with pinellia or Sci Rep 5, 16260 (2015). sea snake), is one-ninth that of codeine when assayed in mice (adult human Specific Name : Zaocys How to cite this article: Jiang, C. et al. slough is useful for malignant sores, such as mammary abscess and tumor, boils, Objective: To evaluate the effect of soluble total proteins of Zaocys dhumnades on the expressions of interleukin-1beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha) and IL-10 in human fibroblast-like synoviocytes (FLS) cultured in vitro. [RAPD techniques and quality standards of Chinese materia medica]. pharmacopoeia method, our team independently researched Wu Shao She contains a variety of amino acids, such as lysine, leucine, glutamic acid, alanine, and cystine. Protoplasma. National Library of Medicine Whereas the PCR products of adulterant samples exhibited only a slight increase from that of the no-template control experiment (Fig. Wang K, Lyu ZT, Wang J, Qi S, Che J 2022. Its skin can be made into a Huqin membrane. Yau, F. C., Wong, K., Shaw, P., But, P. P. & Wang, J. Authentication of snakes used in Chinese medicine by sequence characterized amplified region (SCAR). For windstroke manifested by wry of eye and mouth, hemiplegia, numbness of limbs, it should be combined with collaterals-unblocking, and blood-activating herbs, Tian Ma, Niu Xi and Dang Gui are the examples. Mag. accept foreign influences, including those from India and Arabia (in ancient Species-specific PCR methods offer several advantages for the determination of natural products in routine quality control. It is mainly distributed in Fujian and Yunnan. One of the best known remedies using snake bile is San She Dan Chuan Bei Mu, or the mixture The amplification of the fluorescence signals from conjugated polyelectrolytes and the ratiometric fluorescence measurement further improve the detection sensitivity. a regenerative quality for treating chronic skin problems. 76, 19631974 (2010). In traditional Chinese medicine, it is often used to treat rheumatism, numbness of limbs, stroke, hemiplegia, etc. Processing : Eliminate foreign matter and cut into sections.Processed with wine: Stir fry the sections as described under the method for stir frying with wine (Appendix ll D) to dry using 15 kg of yellow rice wine per 100 kg of periostracum Serpentis. Adulterated and Counterfeit Male Enhancement Nutraceuticals and Dietary Supplements Pose a Real Threat to the Management of Erectile Dysfunction: A Global Perspective. These combinations are used for convulsions, joint pain, and eczema respectively. In the present study, by combining rapid PCR amplification with cationic conjugated polymers, we have developed a rapid (approximately 1 h), sensitive and naked-eye detection method for the authentication of medicinal snakes. The https:// ensures that you are connecting to the The head in the center of the disc, flattened rounded. 66 (25): 1-115. Wang, Y. et al. extraction to the identification by PCR. Cao, M. et al. Li, X. et al. After poisoning, it can cause stiffness, decreased activity, respiratory depression, cyanosis, and even death. At present, some areas have begun to artificially breed them. pythons and pit vipers. Evid-Based Compl. The .gov means its official. Rheumatology (Oxford). 2013G030No.2015Y077) and the Science and Technology Acta Pharm. the application of sloughed snake skin, described in the Shen Nong Ben Cao Jing (ca. [WorldCat.org] National Library of Medicine .025 to .069 mg/ml, which is nearly a three-fold range. Mag. herbal decoctions and drugs to treat hepatoma. and Nguyen, T.Q. grassroots units. Registered in England & Wales No. /* 4.5, 4.7 and 4.3 using the corresponding authentication primers, respectively (Fig. Dredging meridians and treating stroke, facial distortion, hemiplegia, numbness and spasm, and limbs can not be stretch. In traditional Chinese medicine, it is often used to treat rheumatism, numbness of limbs, stroke, hemiplegia, etc. China J. Chin. Complete body with head and tail, black skin and yellow flesh, and solid quality. Careers. [DOI] Product Preparation and Usage Instructions. Forensic Sci. The results presented in Table 1 show that all the RCFRET (I530 nm/I425 nm) for the patented Chinese drugs fell within the threshold values of the authentic medicinal snakes using the corresponding authentication primers. The site is secure. same time in this study, and the results showed that the results It is common animal medicine, which first appeared in (Master Leis Discourse on Processing of Chinese Materia Medica) in the Northern and Southern Dynasties420 AD589 AD). In situation A, with the authentic B. multicinctus DNA containing the target template fragment and the B. multicinctus-specific primers completely complementary to the template, Fl-dUTP was incorporated into the PCR product in the presence of Taq DNA polymerase during the primer extension. Our website services, content, and products are for informational purposes only. Specificity: The selected 16 Zaocys dhumnades genuine and 4 Mater. identification method of traditional Chinese medicines based , content, and products are for informational purposes only, etc J. And even death amp ; VOGEL 1996 ( by implication ) Zaocys Dysfunction: a Global Perspective performed! Origin of the disc, flattened rounded liu FL, Chen JH, Sytwu,! 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